Preparation of Sensitive and Specific Oligonucleotide Probes Tailed Using Terminal Transferase and dITP
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چکیده
منابع مشابه
Preparation of sensitive and specific oligonucleotide probes tailed using terminal transferase and dITP.
An oligonucleotide probe tailed with deoxyadenosine-5'-triphosphate or deoxythymine-5'-triphosphate is detectable with high sensitivity, but has a major drawback--the tail co-hybridizes specifically to complementary sequences. This can be a problem when screening cDNA clones that contain poly(dA) sequences. While it is possible to mask the cDNA tail with unlabeled poly(dA) or poly(A) oligonucle...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00283st06